abstract：:The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...

journal_title：Glycobiology

authors： Ono K,Hattori H,Takeshita S,Kurita A,Ishihara M

更新日期：1999-07-01 00:00:00

abstract：:trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to a terminal beta-galactose in glycoproteins and glycolipids. This enzyme, originally identified in Trypanosoma cruzi, belongs to a large family of proteins. Some members of the family lac...

journal_title：Glycobiology

authors： Cremona ML,Campetella O,Sánchez DO,Frasch AC

更新日期：1999-06-01 00:00:00

abstract：:CMP-beta-N-acetylneuraminic acid (CMP-neuNAc) is the substrate for the sialylation of glycoconjugates by sialyltransferases in microbes and higher eukaryotes. CMP-neuNAc synthetase catalyzes the formation of this substrate, CMP-neuNAc, from CTP and neuNAc. In this report we describe the purification of CMP-neuNAc synt...

journal_title：Glycobiology

authors： Vionnet J,Concepcion N,Warner T,Zapata G,Hanover J,Vann WF

更新日期：1999-05-01 00:00:00

abstract：:Since plants are emerging as an important system for the expression of recombinant glycoproteins, especially those intended for therapeutic purposes, it is important to scrutinize to what extent glycans harbored by mammalian glycoproteins produced in transgenic plants differ from their natural counterpart. We report h...

journal_title：Glycobiology

authors： Cabanes-Macheteau M,Fitchette-Lainé AC,Loutelier-Bourhis C,Lange C,Vine ND,Ma JK,Lerouge P,Faye L

更新日期：1999-04-01 00:00:00

abstract：:The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity...

journal_title：Glycobiology

authors： Mechref Y,Chen P,Novotny MV

更新日期：1999-03-01 00:00:00

abstract：:Elevated ammonium concentrations in the medium of cultivated cells have been shown to increase the intracellular levels of uridine-5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) and uridine-5'-diphospho-N-acetylgalactosamine (UDP-GalNAc; Ryll et al., 1994). These sugar nucleotides are substrates for glycosyltransferase...

journal_title：Glycobiology

authors： Gawlitzek M,Papac DI,Sliwkowski MB,Ryll T

更新日期：1999-02-01 00:00:00

abstract：:To study human alpha1,3/1,4fucosyltransferase (Fuc-TIII) as an alpha1,3 fucosyltransferase, we constructed two cell clones, C127-FT and C127-T-FT, by transfecting cDNA in parental (C127) or Polyoma T antigen expressing (C127-T) mouse cells, respectively. Both C127-FT and C127-T-FT clones express high levels of a fucos...

journal_title：Glycobiology

authors： Valli M,Bardoni A,Trinchera M

更新日期：1999-01-01 00:00:00

abstract：:The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...

journal_title：Glycobiology

authors： Vandersall-Nairn AS,Merkle RK,O'Brien K,Oeltmann TN,Moremen KW

更新日期：1998-12-01 00:00:00

abstract：:Rod and cone photoreceptors project from the outer retinal surface into a carbohydrate-rich interphotoreceptor matrix (IPM). Unique IPM glycoconjugates are distributed around rods and cones. Wheat germ agglutinin (WGA) strongly decorates the rod matrix domains and weakly decorates the cone matrix domains. This study c...

journal_title：Glycobiology

authors： Acharya S,Rayborn ME,Hollyfield JG

更新日期：1998-10-01 00:00:00

abstract：:Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives which were initiated by the Xyl-MU acting as a primer were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xy...

journal_title：Glycobiology

authors： Tazawa T,Takagaki K,Matsuya H,Nakamura T,Sasaki M,Endo M

更新日期：1998-09-01 00:00:00

abstract：:Mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) is a type II transmembrane glycoprotein belonging to the C-type lectin family. Our development of monoclonal antibodies led us to discover that a calcium-dependent conformational change is detected by an antibody (termed mAb LOM-11) and tha...

journal_title：Glycobiology

authors： Hosoi T,Imai Y,Irimura T

更新日期：1998-08-01 00:00:00

abstract：:P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...

journal_title：Glycobiology

authors： Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

更新日期：1998-07-01 00:00:00

abstract：:Pig to human xenotransplantation is considered a possible solution to the prevailing chronic lack of human donor organs for allotransplantation. The Galalpha1,3Gal determinant is the major porcine xenogeneic epitope causing hyperacute rejection following human antibody binding and complement activation. In order to ch...

journal_title：Glycobiology

authors： Bäcker AE,Holgersson J,Samuelsson BE,Karlsson H

更新日期：1998-06-01 00:00:00

abstract：:The Endo F2gene was overexpressed in E.coli as a fusion protein joined to the maltose-binding protein. MBP-Endo F2was found in a highly enriched state as insoluble, inactive inclusion bodies. Extraction of the inclusion bodies with 20% acetic acid followed by exhaustive dialysis rendered the fusion protein active and ...

journal_title：Glycobiology

authors： Reddy A,Grimwood BG,Plummer TH,Tarentino AL

更新日期：1998-06-01 00:00:00

abstract：:Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal orig...

journal_title：Glycobiology

authors： Cornillot JD,Pontet M,Dupuy C,Chadli A,Caron M,Joubert-Caron R,Bourin P,Bladier D

更新日期：1998-05-01 00:00:00

abstract：:From a systematic search of the UniGene and dbEST databanks, using human beta 4-galactosyltransferase (beta 4GalT-I), which is recognized to function in lactose biosynthesis, as the query sequence, we have identified five additional gene family members denoted as beta 4GalT-II, -III, -IV, -V, and -VI. Complementary DN...

journal_title：Glycobiology

authors： Lo NW,Shaper JH,Pevsner J,Shaper NL

更新日期：1998-05-01 00:00:00

abstract：:Human alpha-galactosidase A (alpha-Gal A) is the lysosomal glycohydrolase that cleaves the terminal alpha-galactosyl moieties of various glycoconjugates. Overexpression of the enzyme in Chinese hamster ovary (CHO) cells results in high intracellular enzyme accumulation and the selective secretion of active enzyme. Str...

journal_title：Glycobiology

authors： Matsuura F,Ohta M,Ioannou YA,Desnick RJ

更新日期：1998-04-01 00:00:00

abstract：:Detailed structural and functional studies over the last decade have led to current recognition of the mycobacterial lipoarabinomannan (LAM) as a phosphatidylinositol anchored lipoglycan with diverse biological activities. Fatty acylation has been demonstrated to be essential for LAM to maintain its functional integri...

journal_title：Glycobiology

authors： Chatterjee D,Khoo KH

更新日期：1998-02-01 00:00:00

abstract：:Sialic acids can be modified by O-acetyl esters at the 7- and/or 9-position, altering recognition by antibodies, lectins and viruses. 9(7)-O-acetylation is mediated by a sialic acid-specific O-acetyltransferase, which has proven difficult to purify. Two groups have recently isolated cDNAs possibly encoding this enzyme...

journal_title：Glycobiology

authors： Shi WX,Chammas R,Varki A

更新日期：1998-02-01 00:00:00

abstract：:The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL rea...

journal_title：Glycobiology

authors： Chen CP,Song SC,Gilboa-Garber N,Chang KS,Wu AM

更新日期：1998-01-01 00:00:00

abstract：:Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...

journal_title：Glycobiology

authors： Gaudin JC,Arar C,Monsigny M,Legrand A

更新日期：1997-12-01 00:00:00

abstract：:Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...

journal_title：Glycobiology

authors： Lutomski D,Fouillit M,Bourin P,Mellottée D,Denize N,Pontet M,Bladier D,Caron M,Joubert-Caron R

更新日期：1997-12-01 00:00:00

abstract：:It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...

journal_title：Glycobiology

authors： Goletz S,Thiede B,Hanisch FG,Schultz M,Peter-Katalinic J,Müller S,Seitz O,Karsten U

更新日期：1997-10-01 00:00:00

abstract：:The Neu1 locus, in the S region of the murine histocompatibility-2 complex, regulates the sialic acid content of several liver lysosomal enzymes. Three alleles, Neu1a, Neu1b, and Neu1c, have been described on the basis of differential sialylation of the enzyme liver acid phosphatase. The Neu1a allele occurs in a small...

journal_title：Glycobiology

authors： Carrillo MB,Milner CM,Ball ST,Snoek M,Campbell RD

更新日期：1997-10-01 00:00:00

abstract：:Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...

journal_title：Glycobiology

authors： Morelle W,Strecker G

更新日期：1997-09-01 00:00:00

abstract：:Glycoinositolphospholipids (GIPLs) were isolated from promastigotes of the lizard parasites Leishmania adleri by phenol/water extraction. Phosphoinositol oligosaccharides were liberated by mild alkaline hydrolysis, purified by gel filtration and high pH anion exchange chromatography, and characterized by methylation a...

journal_title：Glycobiology

authors： Previato JO,Jones C,Wait R,Routier F,Saraiva E,Mendonça-Previato L

更新日期：1997-07-01 00:00:00

abstract：:We have isolated beta-trace protein from cerebrospinal fluid, serum, plasma, and urine samples of normal volunteers and sera and hemofiltrate of patients with chronic renal failure. Blood-derived and urinary beta-trace have significantly higher molecular weights than their cerebrospinal fluid counterpart, the amino ac...

journal_title：Glycobiology

authors： Hoffmann A,Nimtz M,Conradt HS

更新日期：1997-06-01 00:00:00

abstract：:Endo-alpha-D-mannosidase is a processing enzyme which in contrast to other glycosidases involved in the trimming of N-linked oligosaccharides of glycoproteins acts at an internal position by cleaving the linkage between the glucose-substituted mannose and the internal portion of the polymannose unit and thereby provid...

journal_title：Glycobiology

authors： Dairaku K,Spiro RG

更新日期：1997-06-01 00:00:00

abstract：:Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...

journal_title：Glycobiology

authors： Petitou M,Barzu T,Herault JP,Herbert JM

更新日期：1997-04-01 00:00:00

abstract：:We have previously shown that chondroitin 6-sulfotransferase (C6ST) catalyzes transfer of sulfate not only to position 6 of GalNAc residue of chondroitin but also to position 6 of Gal residue of keratan sulfate. In this study, we examined the sulfation of sialyl lactosamine oligosaccharides by C6ST. C6ST catalyzed tra...

journal_title：Glycobiology

authors： Habuchi O,Suzuki Y,Fukuta M

更新日期：1997-04-01 00:00:00

abstract：:Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...

journal_title：Glycobiology

authors： Hileman RE,Smith AE,Toida T,Linhardt RJ

更新日期：1997-03-01 00:00:00

abstract：:The rat liver sinusoidal endothelial cell (LEC) hyaluronan (HA) receptor was previously identified using a photoaffinity HA derivative (J. Biol. Chem., 267, 20451-20456, 1992). Two polypeptides with M(r) = 175,000 and 166,000, were consistently crosslinked, suggesting that the LEC HA receptor is an oligomer. Whether o...

journal_title：Glycobiology

authors： Yannariello-Brown J,Zhou B,Weigel PH

更新日期：1997-02-01 00:00:00

abstract：:Lepidopteran insect cells are used routinely as hosts for foreign glycoprotein expression by recombinant baculoviruses, but the precise nature of their N-glycosylation pathway remains poorly defined. These cells clearly have processing glucosidases and mannosidases that can convert precursors to Man3GlcNAc2 structures...

journal_title：Glycobiology

authors： Jarvis DL,Bohlmeyer DA,Liao YF,Lomax KK,Merkle RK,Weinkauf C,Moremen KW

更新日期：1997-02-01 00:00:00

abstract：:A number of unusual and rare carbohydrates were tested as potential inhibitors of various glycosidases, as well as inhibitors of N-linked oligosaccharide processing. The best inhibitors of several arylglycosidases and of glucosidase I were L-xylulose and L-fructose. Both of these sugars showed some inhibitory activity...

journal_title：Glycobiology

authors： Muniruzzaman S,Pan YT,Zeng Y,Atkins B,Izumori K,Elbein AD

更新日期：1996-12-01 00:00:00

abstract：:Affinity-purified glycopeptides were prepared from Trypanosoma cruzi using the carbohydrate-specific monoclonal antibody WIC29.26. These glycopeptides contain rhamnose, fucose, xylose, and galactose, in the ratio 1:1:2:3. A series of oligosaccharides was released from the glycopeptides by mild acid hydrolysis, while, ...

journal_title：Glycobiology

authors： Haynes PA,Ferguson MA,Cross GA

更新日期：1996-12-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells express only a subset of the glycosyltransferases activities known to exist. They do not express several fucosyltransferases, galactosyltransferases, sialyltransferases or N-acetylglucosaminyltransferases. However, following mutagenesis or transfection with large amounts of DNA, rare ...

journal_title：Glycobiology

authors： Stanley P,Raju TS,Bhaumik M

更新日期：1996-10-01 00:00:00

abstract：:The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...

journal_title：Glycobiology

authors： Parker W,Lateef J,Everett ML,Platt JL

更新日期：1996-07-01 00:00:00

abstract：:The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis. At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels. The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a conce...

journal_title：Glycobiology

authors： Pan YT,Drake RR,Elbein AD

更新日期：1996-06-01 00:00:00

abstract：:The galectin from Geodia cydonium (GCA) had previously been shown to be involved in regulatory mechanisms of cell sorting and adhesion during reaggregation of allogeneic sponge cells. In this contribution the binding specificity of GCA was established to be GalNAc alpha 1-3GalNAc beta as structural component of Forssm...

journal_title：Glycobiology

authors： Hanisch FG,Baldus SE,Kümmel TA

更新日期：1996-04-01 00:00:00

abstract：:We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...

journal_title：Glycobiology

authors： Etchison JR,Freeze HH

更新日期：1996-03-01 00:00:00